DeepProposals: Hunting Objects and Actions by Cascading Deep Convolutional Layers

In this paper, a new method for generating object and action proposals in images and videos is proposed. It builds on activations of different convolutional layers of a pretrained CNN, combining the localization accuracy of the early layers with the high informative-ness (and hence recall) of the later layers. To this end, we build an inverse cascade that, going backward from the later to the earlier convolutional layers of the CNN, selects the most promising locations and refines them in a coarse-to-fine manner. The method is efficient, because i) it re-uses the same features extracted for detection, ii) it aggregates features using integral images, and iii) it avoids a dense evaluation of the proposals thanks to the use of the inverse coarse-to-fine cascade. The method is also accurate. We show that our DeepProposals outperform most of the previously proposed object proposal and action proposal approaches and, when plugged into a CNN-based object detector, produce state-of-the-art detection performance.Comment: 15 page

Pelaksanaan Program Inisiasi Menyusu Dini Di Wilayah Kerja Puskesmas Lubuk Buaya Kecamatan Koto Tangah

Inisiasi menyusu dini (IMD) atau early latch on adalah kesempatan yang diberikan kepada bayi segera setelah lahir dengan cara meletakkan bayi di perut ibu, kemudian dibiarkan untuk menemukan puting susu ibu dan menyusu hingga puas. Tujuan: Mendeskripsikan pelaksanaan program IMD di wilayah kerja Puskesmas Lubuk Buaya kecamatan Koto Tangah. Metode: Penelitian ini merupakan evaluation research dengan pendekatan kualitatif menggunakan strategi management-oriented systems models, dengan 4 informan utama dan 9 informan triangulasi. Pengumpulan data dilakukan dengan wawancara mendalam, dokumentasi dan observasi. Hasil: Bidan sudah cukup berkualitas dan kompeten dalam pelaksanaan IMD, tidak ada perencanaan dana IMD secara khusus karena IMD merupakan pelayanan persalinan, sarana dan prasarana sudah cukup baik, perencanaan kegiatan IMD sudah cukup baik dilakukan saat ibu memeriksakan kehamilan dan di kelas ibu hamil, pengorganisasian program IMD belum optimal masih ada yang belum tersosialisasikan secara khusus, pelaksanaan kegiatan IMD belum cukup baik, bidan tidak melaksanakan IMD sesuai protap, monitoring dan evaluasi pelaksanaan program IMD langsung dalam bentuk pencatatan dan pelaporan. Simpulan: Bidan belum mematuhi protap IMD sehingga perlu diberikan motivasi agar melaksanakan sesuai protap dan evaluasi keseluruhan program IMD oleh Puskesmas Lubuk Buaya

Cloning, heterologous expression and characterisation of a recombinant cellobiohydrolase from Humicola insolens ATCC16454 in Pichia pastoris

A cellobiohydrolase gene from the thermophilic fungus Humicola insolens ATCC 16454 was expressed in the methylotrophic yeast Pichia pastoris X-33, and the biochemical properties of the recombinant protein were characterised. The full-length cDNA of the cellobiohydrolase gene avi2 was cloned into the P. pastoris expression vector pPICZαC and expressed extracellularly as a recombinant cellobiohydrolase protein with a molecular weight of approximately 52.3 kDa. The purified recombinant Avi2 enzyme displayed an optimal activity at 50°C and was found stable between temperatures of 30°C and 60°C. The optimal pH of the enzyme was pH 5.0. More than 80% of the enzyme activity was retained at pH values ranging from pH3.0 to pH9.0. Recombinant Avi2 enzyme showed its highest activity towards the substrates Avicel (0.075 U mg-1) and Sigmacell-cellulose (0.018 U mg-1). Very low or undetectable hydrolysis was observed with cellobiose and filter paper. Metal ions, such as Mn2+, Co2+, and Ba2+, increased the activity of the recombinant enzyme. Manganese ions caused the highest increase in activity of approximately 1.38-fold compared to the control assay. Other ions such as Pd2+, Cu2+, Zn2+, Fe2+, and SDS, however, inhibited Avi2 enzyme activity. Interestingly, this recombinant enzyme showed high pH stability when it was incubated in either acidic or basic solutions

Pregnant women in four low-middle income countries have a high prevalence of inadequate dietary intakes that are improved by dietary diversity

Background: Up-to-date dietary data are required to understand the diverse nutritional challenges of pregnant women living in low-middle income countries (LMIC). To that end, dietary data were collected from 1st trimester pregnant women in rural areas of Guatemala, India, Pakistan, and Democratic Republic of the Congo (DRC) participating in a maternal lipid-based nutrient supplement (LNS) Randomized Controlled Trial to examine dietary diversity (DD), usual group energy and nutrient intakes, and prevalence of inadequate dietary intakes.Methods: Two 24-h dietary recalls were conducted in ~240 pregnant women/site (total n = 966) prior to 12-week gestation. Adequate DD was assessed, i.e., ≥5 major food groups consumed within the past 24 h. Median, Q1, Q3 intakes (without LNS) of energy, macronutrients, 12 micronutrients, and phytate were examined. The at risk prevalence of inadequate intakes were based on international guidelines for pregnant women.Results: Dietary patterns varied widely among sites, with adequate DD reported: 20% (Pakistan), 25% (DRC), 50% (Guatemala), and 70% (India). Significantly higher intakes of most key nutrients were observed in participants with adequate DD. More than 80% of women in all sites had inadequate intakes of folate, vitamin B12, and choline, and \u3e80% of women in India and DRC also had inadequate intakes of calcium, thiamine, riboflavin, and vitamin B6.Conclusions: Our data highlight the likely need for micronutrient supplementation in pregnancy, specifically multi-micronutrient interventions, and support the value of increasing DD as part of sustainable long-term nutrition programs for women of reproductive age in these poor rural settings in LMIC

Repeat 24-hour recalls and locally developed food composition databases: a feasible method to estimate dietary adequacy in a multi-site preconception maternal nutrition RCT.

Background: Our aim was to utilize a feasible quantitative methodology to estimate the dietary adequacy of \u3e900 first-trimester pregnant women in poor rural areas of the Democratic Republic of the Congo, Guatemala, India and Pakistan. This paper outlines the dietary methods used. Methods: Local nutritionists were trained at the sites by the lead study nutritionist and received ongoing mentoring throughout the study. Training topics focused on the standardized conduct of repeat multiple-pass 24-hr dietary recalls, including interview techniques, estimation of portion sizes, and construction of a unique site-specific food composition database (FCDB). Each FCDB was based on 13 food groups and included values for moisture, energy, 20 nutrients (i.e. macro- and micronutrients), and phytate (an anti-nutrient). Nutrient values for individual foods or beverages were taken from recently developed FAO-supported regional food composition tables or the USDA national nutrient database. Appropriate adjustments for differences in moisture and application of nutrient retention and yield factors after cooking were applied, as needed. Generic recipes for mixed dishes consumed by the study population were compiled at each site, followed by calculation of a median recipe per 100 g. Each recipe’s nutrient values were included in the FCDB. Final site FCDB checks were planned according to FAO/INFOODS guidelines. Discussion: This dietary strategy provides the opportunity to assess estimated mean group usual energy and nutrient intakes and estimated prevalence of the population ‘at risk’ of inadequate intakes in first-trimester pregnant women living in four low- and middle-income countries. While challenges and limitations exist, this methodology demonstrates the practical application of a quantitative dietary strategy for a large international multi-site nutrition trial, providing within- and between-site comparisons. Moreover, it provides an excellent opportunity for local capacity building and each site FCDB can be easily modified for additional research activities conducted in other populations living in the same area